Variation in size and shape of epidermal cells and stomata is a taxonomically important tool in the delimitation of taxa

Figure 2. Light micrographs (LM) of the foliar anatomy of Persicaria spp. A. Spheroidal trichome and paracytic stomata in P. barbata(1000X). B. Four-celled peltate trichome in P.barbata (400X). C. Unicellular eglandular trichomes in P. barbata (1000X). D. One-celled
peltate trichome in P. chinensis (1000X). E. Anisocytic, paracytic and staurocytic stomata and capitate trichomes in P. glabra (200X). F.Unicellular trichomes in P. stagnina (200X). G. Bunch of unicellular trichomes with swollen base in P. stagnina (1000X). H. Two-celled peltate trichome in P. stagnina (400X). I. Short cone shaped eglandular trichomes in P.orientalis at high focus (1000X). J. Glandular trichomes at low focus in P. orientalis (100X). K. Spheroidal trichome in P.orientalis (1000X). L. Two-celled trichome of P. tenella (400X). M. Pericytic stomata in P. amphibia (400X). N. Paracytic stomata and one-celled peltate trichome in P. posumbu (400X). O. Two-celled peltate trichome with striations in P. chinensis (400X).

Taxonomic significance of leaf epidermal anatomy of selected Persicaria Mill. species of family Polygonaceae from Pakistan

Yasmin G., Khan M. A., Shaheen N., Hayat M. Q. (2010)

Ghazalah Yasmin, Mir Ajab Khan, Nighat Shaheen, Muhammad Qasim Hayat,
Quaid-i-Azam University Islamabad, Pakistan.

African Journal of Biotechnology 9(25): 3759-3768 –

file:///C:/Users/wille/Downloads/82483-Article%20Text-198241-1-10-20121022.pdf

Leaf epidermal anatomy of selected Persicaria Mill. species of the family Polygonaceae revealed variation in size and shape of epidermal cells, stomata, glandular and non glandular trichomes. This study proves to be taxonomically important tool in the delimitation of taxa.

Epidermal cell shapes are variable but mostly polygonal. Five different stomatal patterns are reported for Persicaria Mill. Two types of non glandular trichomes are observed only in P. barbata, P. stagnina and P. orientalis which serve as their distinguishing characters. Glandular trichomes are 1, 2 and 4-celled peltate, capitate and spheroidal. Cluster analyses elucidate relationship among different taxa by utilization of leaf epidermal characters.

Stomata in Abutilon (Malvaceae)

 

 

Implication of foliar epidermal features in the taxonomy of Abutilon Mill. (Malvaceae)

by Shaheen N., Khan M. A., Yasmin G., Hayat M. Q., Ahmad M., Zafar M., Jabeen A. ( 2009)

Nighat Shaheen 1 *, Mir Ajab Khan 1 , Ghazalah Yasmin 1 , Muhammad Qasim Hayat 1 , Mushtaq Ahmad 1 , Muhammad Zafar 1 and Asma Jabeen 2

1Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan.

2 Environmental Science Department, Fatima Jinnah Women University Rawalpindi, Pakistan.

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in Journal of Medicinal Plants Research 3(12): 1002-1008

http://www.academicjournals.org/article/article1380523632_Shaheen%20et%20al.pdf

The micro morphology of both adaxial and abaxial leaf surfaces of seven species of the genus Abutilon Mill. (Malvaceae) viz., Abutilon bidentatum A. R. rich, Abutilon fruticosum Guill. & Perr., Abutilon indicum (L.) Sweet, Abutilon molle (Wild.) Sweet, Abutilon muticum (Del. Ex. DC,) Sweet, Abutilon pakistanicum Jafri & Ali and Abutilon theophrasti Medic was investigated using light microscopy to explore and evaluate the taxonomic implication of foliar epidermal features particularly that of glandular and eglandular trichomes.

Leaves of all the seven species were observed to be amphistomatic and amphitrichomic. The ordinary epidermal cells were commonly polygonal with straight walls, or less often irregular in shape, with slightly undulating walls (abaxial surface of Abutilon muticum and Abutilon theophrastii).

Two types of stomata, anisocytic and diacytic were generally more concentrated on abaxial surface. An indumentum of variable density and texture formed of wide variety of glandular and eglandular trichomes on both surfaces was characteristic of the genus. Seven diverse morphological forms of foliar trichomes were recorded. The trichome complements of each of the seven species studied were unique and thus, can easily be used as an important supportive taxonomic character to delimit each.

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Though the leaves are amphistomatic and amphitrichomic, the stomata and trichomes were observed to be more concentrated on the abaxial surface. Largest stomatal complex was observed on abaxial leaf surface of Abutilon bidentatum, 45 (55 ± 3.53) 65 × 25 (33.2 ± 3.12) 41 µm.

Stomata in Abutilon Mill. (Malvaceae)

Photo credit: Google

Abutilon fruticosum Guill. & Perr.

Implication of foliar epidermal features in the taxonomy of Abutilon Mill. (Malvaceae)

by Shaheen N. , Khan M. A., Yasmin G., Hayat M. Q., Ahmad M., Muhammad Zafar M., Jabeen A. (2009)

Nighat Shaheen¹, Mir Ajab Khan¹, Ghazalah Yasmin¹, Muhammad Qasim Hayat¹, Mushtaq Ahmad¹, Muhammad Zafar¹ and Asma Jabeen^2
1Department of Plant Sciences, Quaid-i-Azam University Islamabad, Pakistan.

²Environmental Science department, Fatima Jinnah Women University Rawalpindi, Pakistan.

===============

in J. Med. Plants Res. 3(12): 1002-1008 – ISSN: 1996-0875 – 

http://www.academicjournals.org/journal/JMPR/article-abstract/5E2A88B15428

http://www.academicjournals.org/journal/JMPR/article-full-text-pdf/5E2A88B15428

Abstract

The micro morphology of both adaxial and abaxial leaf surfaces of seven species of the genus Abutilon Mill. (Malvaceae) viz., Abutilon bidentatum A. R. rich, Abutilon fruticosum Guill. & Perr., Abutilon indicum (L.) Sweet, Abutilonmolle (Wild.) Sweet, Abutilon muticum (Del. Ex. DC,) Sweet, Abutilon pakistanicum Jafri & Ali and Abutilon theophrasti Medic was investigated using light microscopy to explore and evaluate the taxonomic implication of foliar epidermal features particularly that of glandular and eglandular trichomes.

Leaves of all the seven species were observed to be amphistomatic and amphitrichomic. The ordinary epidermal cells were commonly polygonal with straight walls, or less often irregular in shape, with slightly undulating walls (abaxial surface of Abutilon muticum and Abutilon theophrastii).

Two types of stomata, anisocytic and diacytic were generally more concentrated on abaxial surface.

An indumentum of variable density and texture formed of wide variety of glandular and eglandular trichomes on both surfaces was characteristic of the genus. Seven diverse morphological forms of foliar trichomes were recorded. The trichome complements of each of the seven species studied were unique and thus, can easily be used as an important supportive taxonomic character to delimit each.

Stomata in Aconogonon and Bistorta (Polygonaceae)

Photo credit: Google

Aconogonon divaricatum1UME.jpg

 

Micromorphological Investigation of Foliar Anatomy of Genera Aconogonon and Bistorta of Family Polygonaceae.

by Yasmin G., Khan M. A., Shaheen N., Hayat M. Q. (2009)

Ghazalah Yasmin, Quaid-i-Azam University (Islamabad, Pakistan)

Nighat Shaheen, University of Sussex (Brighton, United Kingdom)

• 

  Muhammad Azim Khan, Khyber Pakhtunkhwa Agricultural University, Peshawar, Pakistan

Muhammad Qasim Hayat, National University of Sciences and Technology, Islamabad · Department of Plant Biotechnology, Pakistan

 

in Int. J. Agric. Biol., 11: 285–289 –

http://agris.fao.org/agris-search/search.do?recordID=PK2009001396

Abstract

Leaf epidermal studies have been carried out on six species belonging to two genera of the family Polygonaceae.

The use of light microscopy has made possible in depth to study leaf surface features such as shape of epidermal cells, stomatal pattern, their distribution on adaxial and abaxial leaf surface and trichomes types.

Epidermal cell shapes are variable but generally polygonal.

Six different stomatal patterns are reported for Aconogonon (Meisn.) Reichenb. and Bistorta Adans.

Variation among glandular and non-glandular trichomes was also noted.

Cyclocytic stomata are recorded first time in Aconogonon alpinum (All.) Schur.

This anatomical study has taxonomic importance, on the basis of which identification keys are prepared.

Stomatal variations in the genus Artemisia

Fig. 1: Stomatal variations in Artemisia by means of SEM; A, A. absinthium; B, A. biennis; C, A. japonica; D, A. macrocephala; E, A. persica; F, A. santolinifolia; G, A. scoparia; H, A. stricta; I, A. leucotrichum (Scale bar = 10 μm)

Taxonomic implications of foliar epidermal characteristics with special reference to stomatal variations in the genus Artemisia (Asteraceae).

by Hayat M.Q., Ashraf M., Jabeen S., Shaheen N., Yasmin G., Khan M. A. (2010)

Muhammad Qasim Hayat 

Muhammad Ashraf

Shazia Jabeen

Shaheen N.

GHAZALAH YASMIN

MIR AJAB KHAN

 

in International Journal of Agriculture and Biology: Vol. 12, 221–226 –

https://www.researchgate.net/publication/200781758_Taxonomic_Implications_of_Foliar_Epidermal_Characteristics_with_Special_Reference_to_Stomatal_Variations_in_the_Genus_Artemisia_Asteraceae

Fig. 2: Stomatal variations in Artemisia by means of LM; A A. roxburghiana; B, A. tournefortiana; C, S. stenocephalum; D, A. scoparia; E, A. biennis; F, A. japonica; G, A. biennis; H, A. persica; I, A. absinthium (Scale bar = 10μm)

Abstract

The systematic associations between the individual species of genus Artemisia have been very divisive and need all over revisions and investigations. In continuation to our preceding papers, at this time we account the taxonomic importance of foliar epidermal cells characteristics along with stomatal diversity in the 24 taxa of the genus Artemisia by using light microscopy and scanning electron microscopy.

In addition to previously studied anomocytic and anisocytic stomatal types, here we report four new types of stomata (anomotetracytic, paratetracytic, diacytic & paracytic) first time in the genus Artemisia.

Detail of variations in epidermal cells, of both abaxial and adaxial surfaces, of leaves was also given in relation to the taxonomy of the genus and it was found that most of the Seriphidium spp. have elongated smooth wall cells while other Artemisia spp., have wavy margined irregular shape cells with few exceptions.

This study also indicates that foliar epidermal anatomical characteristics are valuable taxonomic traits, which can be utilized to address the taxonomic issues within the genus.