Stomata in aquatic herbs Monochoria (Pontederiaceae)

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Monochoria vaginalis, (a) Transverse section of leaf through the midrib, (b) Mid rib, (c) Lamina, (d) Para dermal section of epidermis, (e) Stomata enlarged, (f) Raphides under polarized light. Monochoria hastata, (g) Transverse section of leaf through the midrib, (h) Mid rib, (i) Lamina, (j) Para dermal section of epidermis, (k) Stomata enlarged, (l) Raphides under polarized light. Ab: Abaxial side, Ac: Air chamber, Ad: Adaxial side, Mr: Mid rib, Vb: Vascular bundle, Pf: Partition filament, Ph: Phloem, Xy: Xylem, Sc: Sclerenchyma, Ep: Epidermis, Pm: Palisade mesophyll, St: Stomata, AdB: Adaxial bundle, AbB: Abaxial bundle, AbE: Abaxial epidermis, AdE: Adaxial epidermis, Pf: Partition filament, La: Lamina, Lv: Lateral vein, Bs: Bundle sheath, Ra: Raphide, Ec: Epidermal cells, Mt: Mesophyll tissue, Pcr: Prismatic crystals, Aw: Anticlinal wall, S: Stoma, Gc: Guard cell, AdP: Adaxial Palisade, AbP: Abaxial Palisade Scale bars: (a, g) = 1 mm, (b) = 350 μm, (c, d, f, h,i, j, l) = 250 μm, (e, k) = 100 μm

 

Comparative Anatomical Characteristics of Emergent Aquatic Herbs-Monochoria vaginalis (Burm. F.) Presl. and Monochoria hastata Solms. (Pontederiaceae)

by Narayanan K. B., Kaliappan I. (2014)

Kasthuri Bai Narayanan,

Kaliappan_Ilango
Ilango Kaliappan, SRM University, Chennai, India

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in International Journal of Botany 10 (1): 13-23 – DOI: 10.3923/ijb.2014.13.23

https://scialert.net/fulltextmobile/?doi=ijb.2014.13.23

Abstract

The present investigation explores and compares the detailed anatomical features of an emergent aquatic herb of Monochoria vaginalis and Monochoria hastata, belonging to water-hyacinth family, Pontederiaceae. These weedy plants are claimed to be highly nutritious and medicinally valuable among many ethnic communities throughout India. But due to their morphological similarity among the related species of this family, identification and utilization of the plant has been ignored and urbanization has resulted in eradication of the herb on the basis of environmental wet land pollution issues.

Both species exhibits unique hydromorphic anatomical features related to the adaptability of the plant to the aquatic environment. Though both species share most of the anatomical features in common, few differences observed in the leaf anatomy like dorsiventral lamina, brachy-paratetracytic stomata of M. vaginalis and isobilateral lamina and paracytic or cytocytic stomata of M. hastata will provide valuable information for the plant identification.

Even though both the species share common morphological features in common which make them difficult to identify, the microscopical variations observed from the current report helps in identification and will meet the demand of standardization requirements of medicinal herbs.


 

Epidermal cells and stomata
Monochoria vaginalis: Para dermal sections were used for studying the stomatal type and epidermal cells. The stomata are present on both upper and lower sides of the lamina. The stomata are brachy-paratetracytic type (Fig. 2d). A stoma has two lateral subsidiary cells and two larger polar subsidiary cells situated on the upper and lower poles of the guard cells. The guard cells are oblong, elliptic measuring 20×50 μm in size. The epidermal cells are fairly thick walled, angular and compact (Fig. 2e).

Monochoria hastata: Stomata occur on both the upper and lower surfaces of the lamina. They are diffused and random in distribution. The stomata are either paracytic or cytocytic, the former being more in frequency (Fig. 2j), the paracytic stoma has two wing like subsidiary cells, one on either side of the guard cells and parallel to the guard cells (Fig. 2k). In the cytocytic type, a stoma is surrounded by four subsidiary cells, two cells being polar and other two being lateral in position.

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Stomata in water hyacinth (Eichhornia crassipes)

 

 

Anatomical studies on water hyacinth (Eichhornia crassipes (Mart.) Solms) under the influence of textile wastewater

by Mahmood Q., Zheng P., Siddiqi M. R., Islam E. u.,  Azim M. R., Hayat Y. (2005)

1Department of Environmental Engineering, Zhejiang University, Hangzhou 310029, China
2Department of Botany, Federal Government Postgraduate College, H-8, Islamabad, Pakistan
3Department of Botany, Forman Christian College Lahore, Pakistan
4Department of Maths/Stat/Computer Science, North Western Frontier Province Agricultural University, Peshawar, Pakistan

 

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in J Zhejiang Univ Sci B. 6(10): 991–998 – doi:  10.1631/jzus.2005.B0991 – 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1390441/

ABSTRACT

Water hyacinth (Eichhornia crassipes (Mart.) Solms) is a prolific free floating aquatic macrohpyte found in tropical and subtropical parts of the earth.

The effects of pollutants from textile wastewater on the anatomy of the plant were studied. Water hyacinth exhibits hydrophytic adaptations which include reduced epidermis cells lacking cuticle in most cases, presence of large air spaces (7~50 μm), reduced vascular tissue and absorbing structures.

Textile waste significantly affected the size of root cells. The presence of raphide crystals was noted in parenchyma cells of various organs in treated plants.


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Leaf epidermis

Epidermal peels of leaves were studied. Trichomes are not observed in epidermis.

Stomata are of paracytic type. The average size of guard cells was calculated to be 7 µm×4 µm, while the average size of the pore was 4 µm×4 µm. The stomata frequency on the upper epidermis was 2.83 mm2 and 3.32 mm2 on the lower epidermis. Thus the leaves are amphistomatic (Figs.(Figs.1010~~11).11).

Stomatal characteristics of epidermis in control and experimental plants are given in Tables Tables55~~6.6. The experimental plants showed significant (P<0.05) reduction in the size of upper and lower epidermal cells, while the stomatal frequency and stomatal index in upper epidermis were not significantly affected.

Two stomatal shapes in Dendrobium (Orchidaceae)

 

 

Existence of Two Stomatal Shapes in the Genus Dendrobium (Orchidaceae) and Its Systematic Significance

by Yukawa T., Ando T., Karasawa K., Hazshimoto K. (1992)

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in American Journal of Botany 79(8): 946-952 –

https://www.jstor.org/stable/2445006?seq=1#page_scan_tab_contents

Abstract

On the basis of the shape and size of the outer stomatal ledge, 153 species of the genus Dendrobium (Orchidaceae) were divided into three groups, i.e., species group I, species group II, and section Grastidium.
Student’s t-test and principal components analysis using seven stomatal characters suggest that the first two groups are quite distinct from each other. The values for section Grastidium, however, are scattered and indicative of heterogeneity.
Our data suggest a high probability that species groups I and II are monophyletic sister groups. However, section Grastidium, in which stomatal diversification has occurred, may be para- or polyphyletic relative to the genus Dendrobium.

Tendency for stomatal index values to increase in the apical direction of the foliar surface

 

 

Stomatal characteristics in different habitat forms of Brazilian species of Epidendrum (Orchidaceae)

by Stancato G. C., Mazzoni-Viveiros S. C., Luchi A. E. (1999)

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in Nordic Journal of Botany 19(3): 271-275 – https://doi.org/10.1111/j.1756-1051.1999.tb01110.x –

https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1756-1051.1999.tb01110.x

Abstract

Brazilian species of the genus Epidendrum, distributed over various habitats, were analysed for epidermal structure to test correlations between habitat form and variation in stomatal parameters.

This study confirmed the tendency for stomatal index values to increase in the apical direction of the foliar surface in this genus. The generally held view that there are a smaller number of stomata on the adaxial surface in amphistomatic leaves was contradicted for E. vesicatum, whose pendent sympodia showed that the adaxial foliar surfaces assume the abaxial position in relation to light incidence.

Correlated evolution between stomatal traits and vein density

 

 

Evolutionary Association of Stomatal Traits with Leaf Vein Density in Paphiopedilum, Orchidaceae

by Zhang S.-B., Guan Z.-J., Sun M., Zhang J.-J., Cao K.-F., Hu H. (2012)

Shi-Bao Zhang,# 1 , 2 Zhi-Jie Guan,# 1 , 3 Mei Sun, 2 Juan-Juan Zhang, 1 Kun-Fang Cao, 2 and Hong Hu 1 , *

1 Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, Yunnan, China,
2 Key Laboratory of Tropical Plant Ecology, Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Kunming, Yunnan, China,
3 State Key Laboratory of Plant Physiology and Biochemistry and College of Agronomy and Biotechnology, China Agricultural University, Beijing, China,
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in PLoS One. 2012; 7(6): e40080 –  doi:  10.1371/journal.pone.0040080 – 

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3386932/

Abstract

Background

Both leaf attributes and stomatal traits are linked to water economy in land plants. However, it is unclear whether these two components are associated evolutionarily.

 

Methodology/Principal Findings

In characterizing the possible effect of phylogeny on leaf attributes and stomatal traits, we hypothesized that a correlated evolution exists between the two. Using a phylogenetic comparative method, we analyzed 14 leaf attributes and stomatal traits for 17 species in Paphiopedilum. Stomatal length (SL), stomatal area (SA), upper cuticular thickness (UCT), and total cuticular thickness (TCT) showed strong phylogenetic conservatism whereas stomatal density (SD) and stomatal index (SI) were significantly convergent. Leaf vein density was correlated with SL and SD whether or not phylogeny was considered. The lower epidermal thickness (LET) was correlated positively with SL, SA, and stomatal width but negatively with SD when phylogeny was not considered. When this phylogenetic influence was factored in, only the significant correlation between SL and LET remained.

Conclusion/Significance

Our results support the hypothesis for correlated evolution between stomatal traits and vein density in Paphiopedilum. However, they do not provide evidence for an evolutionary association between stomata and leaf thickness. These findings lend insight into the evolution of traits related to water economy for orchids under natural selection.

 

Stomata in Habenaria (Orchidaceae)

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Stomatal studies in the genus Habenaria (Orchidaceae)

by Dangat B. T., Gurav R. V. (2016)

Bhaurav Tukaram Dangat, Rajaram Vithoba Gurav,

Shivaji University, Kolhapur, India

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in Richardiana XVI: 232-240 –

http://richardiana.com/pdfRich/Richardiana-vol16-22-Habenaria-stomata.pdf

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Stomatal types and density in orchids

Plate 1: Bulbophyllum sterile Plate 2: Dendrobium aphyllum

 

Stomatal studies in epiphytic orchids

by Abraham C. M., Chacko A. S., Rajendran A., Johnson L. A., Kumar S. (2016)

Chinju Merin Abraham1 , Arun Sam Chacko2 , Arathi Rajendran2 , Liji Anna Johnson2 and Shyan Kumar2

1. Lecturer in Botany, Department of Botany, Catholicate College, Pathanamthitta, Kerala- 689 645

2. B.Sc. Botany Students, Department of Botany, Catholicate College, Pathanamthitta

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in Asia Pacific Journal of Research 1 (XLI): 102-105 – ISSN (Print) : 2320-5504 ISSN (Online) : 2347-4793 –

http://apjor.com/downloads/2807201612.pdf

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Plate 3: Oberonia brachyphylla Plate 4: Aerides ringens

ABSTRACT

Leaf anatomy was investigated in six epiphytic orchids under Orchidaceae family. Five species of orchids such as Acampe praemorsa, Aerides ringens, Bulbophyllum sterile, Dendrobium aphyllum and Oberonia brachyphylla were studied.

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Plate 5: Acampe praemorsa

Anomocytic stomata were recorded and stomatal index ranges from 7.69% to 28.6%. The minimal stomatal index was recorded in Dendrobium aphyllum (7.69%) where as maximal is in Acampe praemorsa (28.66%).

The number of stomata in a definite area of leaf varies from plant to plant. Low value of stomatal index is an adaptation of orchid plants to survive in different climatic conditions by conserving water. A detailed study is needed to reveal these differences in stomatal density.