Stomatal ontogeny and morphology in Phaseolus vulgaris in relation to infection structure initiation by Uromyces appendiculatus.
by Terhune T., Allen E. A., Hoch H. C., Wergin W., Erbe E. F. (1991)
Cornell Univ., New York State Agricultural Exp. Stn.
B. Tyler Terhune, University of California, Santa Cruz
Eric A Allen, Natural Resources Canada
William Peter Wergin, Natural Resources Research Institute 
in Can. J. Bot. 69, 477–484. – 10.1139/b91-064
Abstract
The development and morphology of the stomatal complex in Phaseolus vulgaris was examined by light microscopy, scanning electron microscopy, and transmission electron microscopy (TEM).
The outer aperture formed between the stomatal guard cells was bordered by cuticular ledges, 1.2–5.3 μm wide. These were composed of a matrix of electron-dense fibrils supporting an autofluorescent amorphous outer layer, homologous to the cuticle. This layer of cuticle lined the ventral walls of the guard cells and extended into the substomatal chamber.
During stomatal development, as the guard cells separated, the outer cuticular layer covering the incipient aperture stretched and split, forming stomatal lips. These lips, 0.2–1.4 μm wide, were oriented horizontally, upright, and folded back from the ledge in TEM thin sections.
In cryopreserved stomata, the lips were generally oriented upright regardless of whether the outer aperture was open or closed. Previous studies have implicated that stomatal lips may function to signal appressorium formation in urediniospore germlings of Uromyces appendiculatus.
This study indicated that dimensions of the lips were within the parameters required to induce appressorium formation on artificial membranes. Other components of the stomatal architecture may also be involved in the induction of appressorium formation.
PLANT STOMATA ENCYCLOPEDIA 