Tag: Lathouwers J. E.

The generation of transgenic sugar beets from stomatal guard cells.

 

A high efficiency technique for the generation of transgenic sugar beets from stomatal guard cells.

by Hall R. D.,GetMedia

Riksen-Bruinsma T.,GetMedia-1

Weyens G. J.,

Rosquin I. J.,

Denys P. N.,

Evans I. J.,

Lathouwers J. E.,

Lefèbvre M. P.,

Dunwell J. M.,biosci-dunwell

van Tunen A.,

Krens F. A.GetMedia

 (1996b)

in Nat. Biotechnol. 14, 1133–1138. –

CrossRefMedlineWeb of Science

http://www.ncbi.nlm.nih.gov/pubmed/9631066?dopt=Abstract

Abstract

An optimized protocol has been developed for the efficient and rapid genetic modification of sugar beet (Beta vulgaris L.).

A polyethylene glycol-mediated DNA transformation technique could be applied to protoplast populations enriched specifically for a single totipotent cell type derived from stomatal guard cells, to achieve high transformation frequencies.

Bialaphos resistance, conferred by the pat gene, produced a highly efficient selection system. The majority of plants were obtained within 8 to 9 weeks and were appropriate for plant breeding purposes. All were resistant to glufosinate-ammonium-based herbicides.

Detailed genomic characterization has verified transgene integration, and progeny analysis showed Mendelian inheritance.