Tag: Jessica A. S. Barros

Manipulation of the tonoplastic organic acid transporter impacted mitochondrial metabolism, while the overall stomatal and photosynthetic capacity were unaffected

 

 

Impaired Malate and Fumarate Accumulation Due to the Mutation of the Tonoplast Dicarboxylate Transporter Has Little Effects on Stomatal Behavior

by Medeiros D. B., Barros K. A., Barros J. A. S., Omena-Garcia R. P., Arrivault S., Sanglard L. M. V. P., Detmann K. C., Silva W. B., Daloso D. M., DaMatta F. M., Nunes-Nesi A., Fernie A. R., Araújo W. L. (2017)

David B. Medeiros, Kallyne Barros, Jessica A. S. Barros, Rebeca P. Omena-Garcia, Stéphanie Arrivault, Lilian Vincis Pereira Sanglard, Kelly C. Detmann, Willian Batista Silva, Danilo M. Daloso, Fabio DaMatta, Adriano Nunes-Nesi, Alisdair R. Fernie, Wagner L Araújo,

Medeiros DB1,2,3, Barros KA1,2, Barros JAS1,2, Omena-Garcia RP1,2, Arrivault S3, Sanglard LMVP2, Detmann KC2, Silva WB1,2, Daloso DM3, DaMatta FM2, Nunes-Nesi A1,2, Fernie AR3, Araújo WL2.

1 Max-Planck Partner Group at the Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-900 Viçosa, Minas Gerais, Brazil.

2 Departamento de Biologia Vegetal, Universidade Federal de Viçosa, 36570-900 Viçosa, Minas Gerais, Brazil.

3 Max Planck Institute of Molecular Plant Physiology, 14476 Potsdam-Golm, Germany.

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in Plant Physiol. 2017 Nov;175(3):1068-1081. doi: 10.1104/pp.17.00971 – Epub 2017 Sep 12 –

https://www.ncbi.nlm.nih.gov/pubmed/28899959

Abstract

Malate is a central metabolite involved in a multiplicity of plant metabolic pathways, being associated with mitochondrial metabolism and playing significant roles in stomatal movements.

Vacuolar malate transport has been characterized at the molecular level and is performed by at least one carrier protein and two channels in Arabidopsis (Arabidopsis thaliana) vacuoles.

The absence of the Arabidopsis tonoplast Dicarboxylate Transporter (tDT) in the tdt knockout mutant was associated previously with an impaired accumulation of malate and fumarate in leaves.

Here, we investigated the consequences of this lower accumulation on stomatal behavior and photosynthetic capacity as well as its putative metabolic impacts. Neither the stomatal conductance nor the kinetic responses to dark, light, or high CO2 were highly affected in tdt plants.

In addition, we did not observe any impact on stomatal aperture following incubation with abscisic acid, malate, or citrate. Furthermore, an effect on photosynthetic capacity was not observed in the mutant lines. However, leaf mitochondrial metabolism was affected in the tdt plants.

Levels of the intermediates of the tricarboxylic acid cycle were altered, and increases in both light and dark respiration were observed.

We conclude that manipulation of the tonoplastic organic acid transporter impacted mitochondrial metabolism, while the overall stomatal and photosynthetic capacity were unaffected.