Pore size regulates operating stomatal conductance, while stomatal densities drive the partitioning of conductance between leaf sides
by Fanourakis D., Giday H., Milla R.,
Ottosen C.-O. (2015)
in Ann. Bot.-London 115: 555–565 – https://doi.org/10.1093/aob/mcu247
https://link.springer.com/article/10.1007/s11099-018-0847-z
Abstract
Methods Stomatal responsiveness to desiccation, gs-related anatomical traits of each leaf side and estimated gs (based on these traits) were determined for 54 introgression lines (ILs) generated by introgressing segments of Solanum pennelli into the S. lycopersicum‘M82’. A quantitative trait locus (QTL) analysis for stomatal traits was also performed.
Key Results A wide genetic variation in stomatal responsiveness to desiccation was observed, a large part of which was explained by stomatal length. Operating gs ranged over a factor of five between ILs. The pore area per stomatal area varied 8-fold among ILs (2–16 %), and was the main determinant of differences in operating gs between ILs. Operating gs was primarily positioned on the abaxial surface (60–83 %), due to higher abaxial stomatal density and, secondarily, to larger abaxial pore area. An analysis revealed 64 QTLs for stomatal traits in the ILs, most of which were in the direction of S. pennellii.
Conclusions The data indicate that operating and maximum gs of non-stressed leaves maintained under stable conditions deviate considerably (by 45–91 %), because stomatal size inadequately reflects operating pore area (R2 = 0·46). Furthermore, it was found that variation between ILs in both stomatal sensitivity to desiccation and operating gs is associated with features of individual stoma. In contrast, genotypic variation in gspartitioning depends on the distribution of stomata between the leaf adaxial and abaxial epidermis.