Stomata in Bergenia (Saxifragaceae)

Photo credit: Researchgate

Figure 9a and 9b. Epidermis with stomata in leaf of Bergenia ciliate (Haw.) Sternb

Pharmacognostic evaluation of Bergenia ciliata (Haw.) Sternb.

by Kahn S. A., Dastagir G., Barkatullah, Ullah S., Ahmad I., Ali U. (2017)

Shafqat Ali Khan, Ghulam Dastagir, Barkatullah, Sami Ullah, Izhar Ahmad and Usman Ali.

in Pure and Applied Biology 6(2): 762-775 – http://dx.doi.org/10.19045/bspab.2017.60081 –

http://www.thepab.org/files/2017/June-2017/PAB-MS-1702029.pdf

maxresdefault
Photo credit Google – Bergenia ciliata [Seeds, leaves & Roots] – Pashanabheda India – https://i.ytimg.com/vi/Qf9MWQUocrA/maxresdefault.jpg
Abstract
The present study was conducted to investigate the Bergenia ciliata (Haw.) Sternb. (Family: Saxifragaceae) for pharmacognostic study including macroscopical and microscopical observations, leaf surface features, Scanning Electron Microscopy and Fluorescence characters.
The macroscopy revealed that rhizome was brown in color, hard in fracture, pleasant in odor and astringent in taste. Leaf was cordate, green in color and indistinct in taste and odour, while microscopy of rhizome and leaf showed typical dicot histological differentiation.
Leaf was amphistomatic having anomocytic stomata on both the epidermises. The quantitative leaf study showed that the stomatal numbers of upper and lower epidermises were (20± 0.7) and (24.2 ± 2.7) (average ± SEM), while stomatal index was (15.6 ± 2.6) and (27.4 ± 3.9) respectively.
Vein islet and vein termination number was (20.6 ± 4.09 and 26.6 ± 5.01), while palisade ratio remained (12.3 ± 3.71). SEM of the powder showed non-glandular trichomes, spiral vessels, parenchymatous tissues and fragments of epidermis with anomocytic stomata.
Fluorescence study indicates different shadows of colors like brown, black, greenish, yellowish, red, brown, dark black, pink etc. that revealed the presence of different fluorescent chemical compounds.
The current research was done for the first time on Bergenia and might be beneficial for its accurate identification, standardization and will give a base for its pharmacognistic applications.

 

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Stomata in Bergenia ciliata (Saxifragaceae)

 

Photo credit: Google

Bergenia ciliata

Pharmacognostic evaluation of Bergenia ciliata (Haw.) Sternb

by Khan S. A., Dastagir G., Ullah B., Ullah S., Ahmad I., Ali U. (2017)

in Pure and Applied Biology. Vol. 6, Issue2, pp. 762-775. – DOI: 10.19045/bspab.2017.60081 

https://www.researchgate.net/publication/317641302_Pharmacognostic_evaluation_of_Bergenia_ciliata_Haw_Sternb

Shafqat_Khan5
Shafqat Ali Khan1*, Department of Botany Islamia College, Peshawar-Pakistan

 

Ghulam Dastagir2, Department of Botany University of Peshawar-Pakistan

Barkat_Ullah3
Barkat Ullah, Islamia College Peshawar ,

Sami Ullah2, Department of Botany University of Peshawar-Pakistan

Izhar Ahmad1, Department of Botany Islamia College, Peshawar-Pakistan

Usman Ali3, Centre of Plant Biodiversity University of Peshawar-Pakistan

1. Department of Botany Islamia College, Peshawar-Pakistan
2. Department of Botany University of Peshawar-Pakistan
3. Centre of Plant Biodiversity University of Peshawar-Pakistan

Abstract

The present study was conducted to investigate the Bergenia ciliata (Haw.) Sternb. (Family: Saxifragaceae) for pharmacognostic study including macroscopical and microscopical observations, leaf surface features, Scanning Electron Microscopy and Fluorescence characters.

The macroscopy revealed that rhizome was brown in color, hard in fracture, pleasant in odor and astringent in taste. Leaf was cordate, green in color and indistinct in taste and odour, while microscopy of rhizome and leaf showed typical dicot histological differentiation.

Leaf was amphistomatic having anomocytic stomata on both the epidermises. The quantitative leaf study showed that the stomatal numbers of upper and lower epidermises were (20± 0.7) and (24.2 ± 2.7) (average ± SEM), while stomatal index was (15.6 ± 2.6) and (27.4 ± 3.9) respectively.

Vein islet and vein termination number was (20.6 ± 4.09 and 26.6 ± 5.01), while palisade ratio remained (12.3 ± 3.71). SEM of the powder showed non-glandular trichomes, spiral vessels, parenchymatous tissues and fragments of epidermis with anomocytic stomata.

Fluorescence study indicates different shadows of colors like brown, black, greenish, yellowish, red, brown, dark black, pink etc. that revealed the presence of different fluorescent chemical compounds.

The current research was done for the first time on Bergenia and might be beneficial for its accurate identification, standardization and will give a base for its pharmacognistic applications.

Stomata in Bignoniaceae

Photo credit: Google

Tecomella undulata

Study of Stomatal Complexes and Appendages of Some Members of Family Bignoniaceae

by Alam N., Wazir K., Razzaq A., Rashid A., Ali U., Hadi F., Iqbal A. (2016)

Noor Alam, Khan Wazir, Abdul Razzaq, Abdur Rashid, Usman Ali, Fazal Hadi, Ajmal Iqbal,

Usman_Ali55
Usman Ali, University of Peshawar, Pakistan
Fazal_Hadi2
Fazal Hadi, University of Peshawar, Pakistan

in JCBPS; Section B; May 2016 – July 2016, Vol. 6, No. 3; 821-827 –

http://www.citefactor.org/article/index/86947/study-of-stomatal-complexes-and-appendages-of-some-members-of-family-bignoniaceae

Abstract:

Bignoniaceae is highly evolved family among the dicotyledons. The reasons for the advancement of the family are solely based on the macro-morphological characteristics. But so far some reliable anatomical characteristics of epidermal emergences and stomatal types of adaxial and abaxial epidermis in 7 species of Bignoniaceae showed that most of the species are hypostomatic i.e having stomata on the lower epidermis while Tecomella undullata is amphistomatic in the investigated plants.

Trichomes found in the investigated species are unicellular, peltate, non-glandular, stellate and tuft of hairs.

The characters showed that the family has both primitive and advanced characters.