Leaf Clearing Protocol to Observe Stomata and Other Cells on Leaf Surface
by Sharma N. (2017)
In bio-101 2017-09-05 – DOI: 0.21769/BioProtoc.2538
In this protocol, leaves are cleared and fixed in an ethanol and acetic acid solution, and mounted in Hoyer’s solution.
The cleared leaves are imaged under differential interference contrast (DIC) microscope. This protocol is beneficial for studying stomata, hair cells, and other epidermal cells in plants.