Epidermal patterning and stomatal development in Gnetales (Gymnospermae)

Epidermal patterning and stomatal development in Gnetales

by Rudall P. J., Rice C. L. (2019)

Paula J. Rudall, Callie L. Rice,

Royal Botanic Gardens, Kew, Richmond, TW9, UK

===

In Annals of Botany, mcz053 – https://doi.org/10.1093/aob/mcz053 –

https://academic.oup.com/aob/advance-article-abstract/doi/10.1093/aob/mcz053/5482537?redirectedFrom=fulltext

Abstract

Background and Aims

The gymnosperm order Gnetales, which has contentious phylogenetic affinities, includes three extant genera (EphedraGnetumWelwitschia) that are morphologically highly divergent and have contrasting ecological preferences: Gnetum occupies mesic tropical habitats, whereas Ephedraand Welwitschia occur in arid environments. Leaves are highly reduced in Ephedra, petiolate with a broad lamina in Gnetum and persistent and strap-like in Welwitschia. We investigate stomatal development and prepatterning stages in Gnetales, to evaluate the substantial differences among the three genera and compare them with other seed plants.

Methods

Photosynthetic organs of representative species were examined using light microscopy, scanning electron microscopy and transmission electron microscopy.

Key Results

Stomata of all three genera possess lateral subsidiary cells (LSCs). LSCs of Ephedra are perigene cells derived from cell files adjacent to the stomatal meristemoids. In contrast, LSCs of Gnetumand Welwitschia are mesogene cells derived from the stomatal meristemoids; each meristemoid undergoes two mitoses to form a ‘developmental triad’, of which the central cell is the guard mother cell and the lateral pair are LSCs. Epidermal prepatterning in Gnetum undergoes a ‘quartet’ phase, in contrast with the linear development of Welwitschia. Quartet prepatterning in Gnetum resembles that of some angiosperms but they differ in later development.

Conclusions

Several factors underpin the profound and heritable differences observed among the three genera of Gnetales. Stomatal development in Ephedra differs significantly from that of Gnetum and Welwitschia, more closely resembling that of other extant gymnosperms. Differences in epidermal prepatterning broadly reflect differences in growth habit between the three genera.

Advertisements

Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

Leave a Reply

Please log in using one of these methods to post your comment:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s