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The effects of temperature and ABA on stomata of Zea mays L.
by Rodriguez J. L., Davies W. J. (1982)
in J. Exp. Bot. 33, 977–987. – doi: 10.1093/jxb/33.5.977 –
The sensitivity of stomata to temperature was dependent upon the technique used to incubate epidermis. Generally, the widest apertures were recorded at around 25°C.
In all experiments, stomata incubated at low (10°C) temperatures on 5.6 × 10−4 M ABA showed wider apertures than those incubated on distilled water. This ABA-stimulated stomatal opening was accompanied by an increase in the intensity of potassium staining in the guard cells. At 25 °C, epidermis incubated on several concentrations of ABA showed some stomatal closure, decreased potassium staining in the guard cells and increased potassium staining in the subsidiary cells.