Stomatal response to environmental factors and growth regulators


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Stomatal Response of in vitro Cultured Plantlets. I. Responses in Epidermal Strips of Chrysanthemum to Environmental Factors and Growth Regulators

by Wardle K. , Short K. C. (1983)

Department of Biology, North East London Polytechnic, England


in Biochemie und Physiologie der Pflanzen 178(8): 619-624 – –  



Abaxial epidermal strips removed from tissue cultured Chrysanthemum plantlets during the light or dark revealed open stomata and the guard cells contained starch.

Closure of the stomata was not induced by incubation of epidermal strips in darkness or by the presence of ABA.

Further stomatal opening was induced by CO2-free air and by kinetin. Following further opening using CO2- free air, the stomata could be induced to exhibit partial closure to their initial level of opening using ABA and darkness.


Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

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