Dithithreitol inhibits blue-light-stimulated stomatal opening

vicia-faba-st-nkramer
Photo credit: Google – Vicia faba (broad vetch) – https://newfs.s3.amazonaws.com/taxon-images-1000s1000/Fabaceae/vicia-faba-st-nkramer.jpg

 

The inhibitor of zeaxanthin formation, dithithreitol, inhibits blue-light-stimulated stomatal opening in Vicia faba.

by Srivastava A., Zeiger E. (1995a)

  • Alaka Srivastava
  • Eduardo Zeiger

in Planta 196, 450-457. –

CrossRef |-

https://link.springer.com/article/10.1007%2FBF00203642

Abstract

Dithiothreitol (DTT), an inhibitor of violaxanthin de-epoxidation and zeaxanthin formation in chloroplasts, inhibited blue-light-stimulated stomatal opening in epidermal peels of Vicia faba L. in a concentration-dependent fashion.

Complete inhibition was observed at 3 mM DTT. The DTT effect was specific for the stomatal response to blue light, and the red-light-stimulated opening, which depends on photosynthetic reactions in the guard cells, was unaffected.

Preirradiation of stomata in epidermal peels with increasing photon fluence rates of red light, prior to an incubation in 10 μmol·m-2·s-1 of blue light and 100 μmol·m-2·s-1 red light, resulted in a DTT-sensitive, blue-light-stimulated opening that was proportional to the fluence rate of the red light pre-treatment.

Guard cells in epidermal peels and guard-cell protoplasts irradiated with red light showed increases in their zeaxanthin content that depended on the fluence rate of red light, or on the incubation time. The increases in zeaxanthin concentration were inhibited by DTT.

The obtained results indicate that zeaxanthin could function as a photoreceptor mediating the stomatal responses to blue light.

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Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

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