Multiplexed isobaric tagging and ABA responsive proteins in stomata

Photo credit: Science Direct

Analysis of abscisic acid responsive proteins in Brassica napus guard cells by multiplexed isobaric tagging.

by Zhu M., Simons B., Zhu N., Oppenheimer D. G., Chen, S. (2010)

Mengmeng Zhua. Brigitte Simonsb. Ning Zhua. David G. Oppenheimera. Sixue Chena

a

Department of Biology, UF Genetics Institute, University of Florida, Gainsville, FL 32610, USA
b
MDS Analytical Technologies (SCIEX), Ontario, Canada L4K 4V8
c
Plant Molecular and Cellular Biology Program, University of Florida, Gainesville, FL 32611, USA
d
Proteomics Division, Interdisciplinary Center for Biotechnology Research, University of Florida, Gainesville, FL 32610, USA

in J. Proteomics 73, 790–805. – doi: 10.1016/j.jprot.2009.11.002 –

PubMed Abstract | CrossRef Full Text | Google Scholar

http://www.sciencedirect.com/science/article/pii/S1874391909003303?via%3Dihub

Abstract

Guard cells, which form stomata on the leaf epidermis, play important roles in plant gas exchange and defense against pathogens.

Abscisic acid (ABA) is a phytohormone that can be induced by drought and leads to stomatal closure.

Guard cells have been a premier model system for studying ABA signal transduction. Despite significant progress on the identification of molecular components in the ABA signaling pathway, our knowledge of the protein components is very limited.

Here, we employ a recently developed multiplexed isobaric tagging technology to identify ABA-responsive proteins in Brassica napus guard cells. A total of 431 unique proteins were identified with relative quantitative information in control and ABA-treated samples.

Proteins involved in stress and defense constituted a major group among the 66 proteins with increased abundance. Thirty-eight proteins were decreased in abundance and fell into several functional groups including metabolism and protein synthesis. Many of the proteins have not been reported as being ABA responsive or involved in stomatal movement.

A large percentage of the protein-coding genes contained ABA-responsive elements.

This study not only established a comprehensive inventory of ABA-responsive proteins, but also identified new proteins for further investigation of their functions in guard cell ABA signaling.

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Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

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