Hydrogen peroxide, pH and stomatal closure.

 

Hydrogen peroxide-induced changes in intracellular pH of guard cells precede stomatal closure.

by Zhang X., Dong F. C., Gao J. F., Song C. P. (2001)

Xiao ZHANG1,2, Fa Cai DONG1, Jun Feng GAO2 and Chun Peng SONG1

1 Department of Biology, Henan University, Kaifeng 475001, China

2 College of Life Sciences, Northwest Sci-Tech University of Agriculture and Forestry, Yangling 712100, China

in Cell Res. 11, 37–43. – doi: 10.1038/sj.cr.7290064 –

PubMed Abstract | CrossRef Full Text | Google Scholar

https://www.nature.com/cr/journal/v11/n1/full/7290064a.html

Abstract

Epidermal bioassay demonstrated that benzylamine, a membrane-permeable weak base, can mimick hydrogen peroxide (H2O2) to induce stomatal closure, and butyric acid, a membrane-permeable weak acid, can partly abolish the H2O2-induced stomatal closure.

Confocal pH mapping with the probe 5-(and-6)-carboxy seminaphthorhodafluor-1-acetoxymethylester (SNARF-1-AM) revealed that H2O2 leads to rapid changes in cytoplasmic and vacuolar pH in guard cells of Vicia faba L, i. e. alkalinization of cytoplasmic areas occur red in parallel with a decrease of the vacuolar pH, and that butyric acid pretreatment can abolish alkalinization of cytoplasmic areas and acidification of vacuolar areas of guard cells challenged with H2O2.

These results imply that the alkalinization of cytoplasm via efflux of cytosol protons into the vacuole in guard cells challenged with H2O2 is important at an early stage in the signal cascade leading to stomatal closure.

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Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

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