Figure 1. Transcriptional Profiling of Stomatal Lineage Cells Isolated by FACS
(A) Cartoon of stages in stomatal development with confocal images of markers used for FACS. Specific reporters used to mark cell stages are ML1p::YFP-RCI2A, epidermal cells (including stomatal lineage cells), gray; SPCHp::SPCH-YFP, stomatal entry, green; MUTEp::nucGFP, commitment, light blue; FAMAp::GFP-FAMA, differentiation, violet; and
E1728::GFP, maturation, purple.
(B) Scheme of cell isolation protocol. Aerial seedling tissues expressing markers were protoplasted and FACS for RNA extraction. Expression profiles of sorted cells were generated using RNA-Seq and ATH1 microarrays (ATH1).
Transcriptome Dynamics of the Stomatal Lineage: Birth, Amplification, and Termination of a Self-Renewing Population
by Adrian J., Chang J., Ballenger C., Bargmann B., Alassimone J., Davies K. A., Lau O. S., Matos J. L., Hachez C., Lanctot A., Vatén A., Birnbaum K. D., Bergmann D. C. (2015)
1 Jessika Adrian, Stanford University, Stanford, CA, USA
1,6 Charles Hachez, Université Catholique de Louvain, Louvain-la-Neuve, Belgium
1,7 Amy Lanctot, University of Washington, Seattle, WA, USA
1 Anne Vatén,Stanford University, Stanford, CA, USA
2 Kenneth D. Birnbaum,New York University, New York, NY, USA
1 Department of Biology, Stanford University, Stanford, CA 94305, USA
2 Biology Department, Center for Genomics and Systems Biology, New York University, New York, NY 10003, USA
3 Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA
4 Present address: Department of Genetics, Stanford Medical School, Stanford, CA 94305, USA
5 Present address: Cibus US LLC, San Diego, CA 92121, USA
6 Present address: Institut des Sciences de la Vie, Université Catholique de Louvain, Louvain-la-Neuve 1348, Belgium
7 Present address: Department of Biology, University of Washington, Seattle, WA 98195, USA
in Developmental Cell 33(1):107-18 · April 2015- DOI: 10.1016/j.devcel.2015.01.025 –
Developmental transitions can be described in terms of morphology and the roles of individual genes, but also in terms of global transcriptional and epigenetic changes. Temporal dissections of transcriptome changes, however, are rare for intact, developing tissues.
We used RNA sequencing and microarray platforms to quantify gene expression from labeled cells isolated by fluorescence-activated cell sorting to generate cell-type-specific transcriptomes during development of an adult stem-cell lineage in the Arabidopsis leaf.
We show that regulatory modules in this early lineage link cell types that had previously been considered to be under separate control and provide evidence for recruitment of individual members of gene families for different developmental decisions.
Because stomata are physiologically important and because stomatal lineage cells exhibit exemplary division, cell fate, and cell signaling behaviors, this dataset serves as a valuable resource for further investigations of fundamental developmental processes.