Photo credit: Caspian J. Env. Sci.
Fig. 3. SEM micrographs of wax and stomata in subspecies and varieties of Quercus castaneifolia: (A) wax on Abaxial surface, (B) wax on Adaxial surface, (C-F) Stomata, (C) Q. castaneifolia subsp. castaneifolia var. castaneifolia, (D) Q. castaneifolia subsp. castaneifolia var. minuta, (E) Q. castaneifolia subsp. aitchisoniana, (F) Q. castaneifolia subsp. undulate, (A) scale bar = 3.75 μm; (B) scale bar = 100 μm; (C-F) scale bar = 15 μm
A revision of chestnut-leaved oak (Quercus castaneifolia C. A. Mey.; Fagaceae) in Hyrcanian Forests of Iran
by Panahi P., Jamzad Z., Pourmajidian M. R., Fallah A., Pourhashemi M. (2011)
in Caspian J. Env. Sci. 2011, Vol. 9 No.2 pp. 145~158
Chestnut-leaved oak (Quercus castaneifolia C. A. Mey) is one of the most important native oaks of Iran distributed in the Hyrcanian Forests. The pure and mixed stands of it cover about 6.5% of these forests. The species represents morphological variations which have resulted in the description of several infraspecific taxa by different authors. Eight taxa were recognized as subspecies and varieties of Q. castaneifolia.
In this survey, a set of quantitative and qualitative micro-morphological characteristics such as type of trichome, number and length of trichome rays, type of epicuticular waxes, type and shape of stomata, shape and sculptural features of pollen exine were studied using light microscopy and scanning electron microscopy.
These characters were analyzed by the discriminant analysis method and combined with the macromorphological characters to designate the variation within the species and to evaluate the existing infraspecific taxa.
Based on our results, the studied taxa are defined into four infraspecific taxa as follows: Q. castaneifolia subsp. castaneifolia var. castaneifolia, Q. castaneifolia subsp. castaneifolia var. minuta, Q. castaneifolia subsp. aitchisoniana, Q. castaneifolia subsp. undulate.
The studied characteristics showed to be diagnostic for identification of the infraspecific taxa.
Description of observed stomata
We identified an elliptical shape of stomata in all examined taxa except in Q. castaneifolia subsp. castaneifolia var. minuta with round stomata (Fig. 3D-F). Stomata were raised above the epidermal surface in all cases. The rims of stomata were entirely covered by epicuticular waxes of smooth layer type in all studied taxa, but the pore was visible.
Quantitative features of trichomes and stomata of the examined taxa are presented in table 7.