CPK6, YEL and stomatal movement.

 

Calcium-dependent protein kinase CPK6 positively functions in induction by yeast elicitor of stomatal closure and inhibition by yeast elicitor of light-induced stomatal opening in Arabidopsis.

by Ye W., Muroyama D., Munemasa S., Nakamura Y., Mori I.C., Murata, Y. (2013)

Wenxiu Ye,

Daichi Muroyama,

Shintaro Munemasa,

Yoshimasa Nakamura,

Izumi C. Mori,

Yoshiyuki Murata

Graduate School of Environmental and Life Science, Okayama University, 1–1–1 Tsushima-Naka, Okayama 700–8530, Japan (W.Y., D.M., S.M., Y.N., Y.M.);

Institute of Plant Science and Resources, Okayama University, Kurashiki, Okayama 710–0046, Japan (I.C.M.)

in Plant Physiol. 163:591–599. – doi: 10.1104/pp.113.224055 –

PubMed Abstract | CrossRef Full Text | Google Scholar –

http://www.plantphysiol.org/content/163/2/591.full.pdf+html

Abstract

Yeast elicitor (YEL) induces stomatal closure that is mediated by a Ca2+-dependent signaling pathway. A Ca2+-dependent protein kinase, CPK6, positively regulates activation of ion channels in abscisic acid and methyl jasmonate signaling, leading to stomatal closure in Arabidopsis (Arabidopsis thaliana).

YEL also inhibits light-induced stomatal opening. However, it remains unknown whether CPK6 is involved in induction by YEL of stomatal closure or in inhibition by YEL of light-induced stomatal opening.

In this study, we investigated the roles of CPK6 in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis.

Disruption of CPK6 gene impaired induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening. Activation by YEL of nonselective Ca2+-permeable cation channels was impaired in cpk6-2 guard cells, and transient elevations elicited by YEL in cytosolic-free Ca2+ concentration were suppressed in cpk6-2 and cpk6-1 guard cells.

YEL activated slow anion channels in wild-type guard cells but not in cpk6-2 or cpk6-1 and inhibited inward-rectifying K+ channels in wild-type guard cells but not in cpk6-2 or cpk6-1. The cpk6-2 and cpk6-1 mutations inhibited YEL-induced hydrogen peroxide accumulation in guard cells and apoplast of rosette leaves but did not affect YEL-induced hydrogen peroxide production in the apoplast of rosette leaves.

These results suggest that CPK6 positively functions in induction by YEL of stomatal closure and inhibition by YEL of light-induced stomatal opening in Arabidopsis and is a convergent point of signaling pathways for stomatal closure in response to abiotic and biotic stress.

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Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

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