Stomatal immunity and ASPARTATE OXIDASE

 

ASPARTATE OXIDASE plays an important role in Arabidopsis stomatal immunity.

by Macho A.P., Boutrot F., Rathjen J.P., Zipfel C. (2012)

, Chinese Academy of Sciences

alberto_macho
Alberto_Macho.png

, The Sainsbury Laboratory

freddy_boutrot
Freddy_Boutrot.png

John P Rathjen

Cyril Zipfel

 

in Plant Physiol. 159:1845–1856. – DOI: 10.1104/pp.112.199810 –

https://www.researchgate.net/publication/228064322_ASPARTATE_OXIDASE_plays_an_important_role_in_Arabidopsis_stomatal_immunity

figure-2-exogenous-na-complements-the-reduced-flg22-triggered-ros-burst-in-fin4-1-a
Figure 2. Exogenous NA complements the reduced flg22-triggered ROS burst in fin4-1 . A, Schematic representation of the de novo NAD biosynthesis pathway. B, Oxidative burst triggered by 100 n M flg22 in – https://www.researchgate.net/profile/Alberto_Macho/publication/228064322/figure/fig2/AS:302285436538882@1449081872945/Figure-2-Exogenous-NA-complements-the-reduced-flg22-triggered-ROS-burst-in-fin4-1-A.png
Abstract
Perception of pathogen-associated molecular patterns (PAMPs), such as bacterial flagellin (or the peptide flg22), by surface-localized receptors activates defense responses and subsequent immunity. In a previous forward-genetic screen aimed at the identification of Arabidopsis (Arabidopsis thaliana) flagellin-insensitive (fin) mutants, we isolated fin4, which is severely affected in flg22-triggered reactive oxygen species (ROS) bursts.
Here, we report that FIN4 encodes the chloroplastic enzyme ASPARTATE OXIDASE (AO), which catalyzes the first irreversible step in the de novo biosynthesis of NAD.
Genetic studies on the role of NAD have been hindered so far by the lethality of null mutants in NAD biosynthetic enzymes. Using newly identified knockdown fin alleles, we found that AO is required for the ROS burst mediated by the NADPH oxidase RBOHD triggered by the perception of several unrelated PAMPs. AO is also required for RBOHD-dependent stomatal closure.
However, full AO activity is not required for flg22-induced responses that are RBOHD independent. Interestingly, although the fin4 mutation dramatically affects RBOHD function, it does not affect functions carried out by other members of the RBOH family, such as RBOHC and RBOHF.
Finally, we determined that AO is required for stomatal immunity against the bacterium Pseudomonas syringae.
Altogether, our work reveals a novel specific requirement for AO activity in PAMP-triggered RBOHD-dependent ROS burst and stomatal immunity.
In addition, the availability of viable mutants for the chloroplastic enzyme AO will enable future detailed studies on the role of NAD metabolism in different cellular processes, including immunity, in Arabidopsis.

 

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Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

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