Stomata in Trigonella spruneriana

 

Comparative leaf epidermis and anatomical study in populations of Trigonella spruneriana (Fabaceae) from Iran

by Ranjbar M., Hajmoradi Z. (2016)

Massoud Ranjbar – 

Zahra Hajmoradi

in Journal of Plant Taxonomy and Geography – Volume 71, Issue 1, 2016 – DOI:10.1080/00837792.2016.1138673 –

http://www.tandfonline.com/doi/abs/10.1080/00837792.2016.1138673

Abstract

The stem and petiole anatomy and also leaf epidermis morphology were studied in 15 populations of Trigonella spruneriana Boiss. in Iran to determine intraspecific variation.

Various anatomical characters were compared, such as number of collenchyma, parenchyma, sclerenchyma fibre layers, and number of vascular bundles, the shape of the cross section outline, density and size of stomata on the adaxial surface of leaflet, stomata type and epidermal cell shape.

A variety of noticeable anatomical features were seen in the studied populations. Some anatomical characters differing in various populations were the shape of cross section outline (square, circle and sinuate), presence or absence of crystals, tannins and percentage of anisocytic stomata (19.6–39.7%).

Type of hairs in all populations was similar. Two types of hair on the stem and petiole surface are papillose–spinulate and glandular.

Moreover, the results of the present study in T. spruneriana as an annual species were compared with the result of our previous study in Trigonella disperma as a perennial species to assess interspecific relationships.Trigonella spruneriana was separated from T. disperma by having more collenchyma cells (2.5–4.5 versus 0.9–2), fewer sclerenchyma cells (1.5–3 versus 2.5–5) and different shape of cross section (square to circle versus sinuate to elliptic).

In addition, these taxa were differentiated from each other by having different densities of crystals and tannins. According to leaf epidermis characters, the two species were separated from each other by having different stomata size and anticlinal cell wall pattern.

Finally, all data were analysed with MVSP ver. 3.1 to determine interspecific and intraspecific diversity.