Stomata in Equisetum.

 

 

Stomata in Equisetum.

by Dayanandan P., Kaufman P. B. (1973)

in Canadian Journal of Botany 51:1555-1564. –

(http://www.nrcresearchpress.com/doi/abs/10.1139/b73-198#.VQg8VxDF-6F)

ABSTRACT

Light and scanning electron microscopic studies and guard cell isolation techniques have confirmed the well-known ridges of the Equisetum stomatal apparatus as belonging to the subsidiary cells.

Hitherto unknown features of the subsidiary cells such as the presence of a concentrated H2SO4-resistant region on the ridges and an interlocking mechanism for the closure of the aperture of the subsidiaries are described. These are presented as further evidence for the differences between the two subgenera of Equisetum.

Boiling in dilute NaOH is shown to be a simple but effective means for the isolation of the guard cells in Equisetum as well as in several other plants with sunken stomata.

Silicification of the outer layer of the epidermis makes cuticular isolation a difficult process by usual methods (treatments with enzymes or cellulose hydrolyzing reagents).

Treatment with concentrated sulfuric acid followed by hydrofluoric acid results in the isolation of the cuticular membranes in Equisetum spp. and in similarly silicified grasses. Involvement of potassium ions in stomatal movements is indicated for two ferns and suggested for two species of Equisetum.

Advertisements

Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

Leave a Reply

Please log in using one of these methods to post your comment:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google+ photo

You are commenting using your Google+ account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

w

Connecting to %s