Light-sheet microscopy of stomata

Preparation of plants for developmental and cellular imaging by light-sheet microscopy

by Ovečka M.Vaškebová L.Komis G., Luptovčiak I., Smertenko A., Šamaj J. (2015)

in Nature Protocols 10, 1234–1247 (2015) –

doi:10.1038/nprot.2015.081 –

Figure 8: Simultaneous imaging of microtubules and F-actin in cotyledon cells of A. thaliana by dual-channel light-sheet microscopy.

Depicted are stomata guard cells (S), stomata meristemoids (arrows), guard mother cells (arrowheads) and pavement cells (stars) in the epidermis of the line expressing microtubule marker TUA5-mCherry and actin marker FABD2-GFP. (a) Microtubules. (b) F-actin filaments. (c) Merged image. Cotyledons were mounted into glass capillaries, as illustrated in Figure 3. Dynamics of F-actin reorganization in the epidermal cells is shown in Supplementary Video 9. Scale bar, 10 μm.

See the text: Nature


Published by

Willem Van Cotthem

Honorary Professor of Botany, University of Ghent (Belgium). Scientific Consultant for Desertification and Sustainable Development.

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