Preparation of plants for developmental and cellular imaging by light-sheet microscopy
in Nature Protocols 10, 1234–1247 (2015) –
Figure 8: Simultaneous imaging of microtubules and F-actin in cotyledon cells of A. thaliana by dual-channel light-sheet microscopy.
Depicted are stomata guard cells (S), stomata meristemoids (arrows), guard mother cells (arrowheads) and pavement cells (stars) in the epidermis of the line expressing microtubule marker TUA5-mCherry and actin marker FABD2-GFP. (a) Microtubules. (b) F-actin filaments. (c) Merged image. Cotyledons were mounted into glass capillaries, as illustrated in Figure 3. Dynamics of F-actin reorganization in the epidermal cells is shown in Supplementary Video 9. Scale bar, 10 μm.
See the text: Nature